FIGURE 2.

Impaired ischemia-induced revascularization in Lkb1^flox/+;Tie2^{Tg/ +} (heterozygous LKB1-KO) mice. A, representative LDBF images showing decreased perfusion in ischemic limbs of Lkb1^flox/+;Tie2^Tg/+ mice. A low perfusion signal (blue) was observed in the ischemic hind limbs of Lkb1^flox/+;Tie2^Tg/+ mice, whereas a high perfusion signal (white to red) was detected in Lkb1^flox/+;Tie2^+/+ on postoperative day 28. B, quantitative analysis of the ischemic/nonischemic LDBF ratio of Lkb1^flox/+;Tie2^+/+ (open circles) and Lkb1^flox/+;Tie2^Tg/+ (filled circles) mice on postoperative days 0, 3, 7, 14, and 28 (n = 6). *, p < 0.05 for Lkb1^flox/+;Tie2^+/+ mice. C, representative immunostaining of nonischemic and ischemic muscle tissues with anti-CD31 antibody (brown) on postoperative day 28. D, quantitative analyses of capillary density in nonischemic and ischemic muscles of Lkb1^flox/+;Tie2^+/+ and Lkb1^flox/+;Tie2^Tg/+ mice on postoperative day 28 (n = 6 in each group). Capillary density was expressed as the number of capillaries/muscle fiber. E, Western blot analysis of phosphorylated eNOS at Ser-1177 (P-eNOS), total eNOS (eNOS), and α-tubulin (Tubulin) in nonischemic and ischemic muscles of Lkb1^flox/+;Tie2^+/+ and Lkb1^flox/+;Tie2^Tg/+ mice on postoperative day 7. F, quantitative analysis of Western blots. *, p < 0.05 versus Lkb1^flox/+;Tie2^+/+ (n = 4 in each group).