Metabolic parameters are normal in Pemt−/− mice fed a chow diet. All data are means ± S.E. A, body weight of 18-week-old male Pemt+/+ and Pemt−/− mice fed the chow diet (n = 4–5). B–F, 16-week-old Pemt+/+ and Pemt−/− mice that had been fed the chow diet (n = 4) were acclimatized for 24 h in an Oxymax Lab Animal Monitoring System and indirect calorimetry measurements were taken every 13 min over a 24-h period. B, food intake (b.w., body weight). C, oxygen consumption. D, respiratory exchange ratio (RER). E, hepatic lipids from male Pemt+/+ and Pemt−/− mice (n = 4–5) were quantified by gas chromatography. C, cholesterol; CE, cholesteryl ester; TG, triacylglycerol. F, glucose tolerance test: chow fed mice (n = 4–5) were injected intraperitoneally with 2 g/kg body weight of glucose, and blood glucose was measured between 15 and 120 min later.