FIGURE 4.

Reduced neutral lipid storage in gastrocnemius muscle of HF diet-fed Pemt^−/− mice. Whole gastrocnemius muscles were removed from HF diet-fed Pemt^+/+ and Pemt^−/− mice that had been fasted overnight. A, neutral lipids (C, cholesterol; CE, cholesteryl ester; and TG, triacylglycerol) were quantified by gas chromatography; *, p < 0.05. B, the relative levels of mRNA-encoding genes involved in fatty acid metabolism were determined by real-time quantitative PCR. Data are relative to cyclophilin mRNA in the same sample (n = 4). *, p < 0.05. PGC1α, PPARγ co-activator; UCP, uncoupling protein; MCD1, malonyl-CoA decarboxylase 1; CPT1, carnitine palmitoyl-CoA transferase 1; ACC, acetyl-CoA carboxylase. C, transmission electron microscope images of mitochondria (some indicated by arrows) in gastrocnemius muscle (10,000-fold magnification). D, the size of individual mitochondria (n = 70–100) was quantified using ImageJ software and is displayed using a box and whiskers plot (p < 0.05).