Attenuated weight gain, increased oxygen consumption, and improved glucose tolerance in Pemt+/+ mice fed the choline-deficient diet. Data are means ± S.E. 8-week-old male Pemt+/+ mice (n = 6) were fed a choline-supplemented (CS) or choline-deficient (CD) diet for 12 weeks. A, weight of the mice was measured at indicated times (p < 0.01). B, glucose tolerance test: 2 g of glucose/kg of body weight was administered by intraperitoneal injection, and blood glucose was measured after 15, 30, 60, and 120 min (n = 6; p < 0.0001). C, oxygen consumption (p < 0.0001) was measured in Pemt+/+ mice that had been fed the CS or CD diet for 9 weeks. D, weight gain of 8-week-old male Pemt+/+ and Pemt−/− mice that had been fed either the HF diet or the HFCS diet for 10 weeks after a 10-week HF/CS feeding period (n = 5–8).