15d-PGJ2 accumulates in nuclei. A, chemical structures of 15d-PGJ2, CAY 10410 (9,10-dihydro-15-deoxy-Δ12,14-prostaglandin J2) and LMB. The asterisks indicate the reactive C-atoms in 15d-PGJ2 and LMB. B, HeLa-cells were treated with 10 μm 15d-PGJ2-biotin for 1 h, fixed, and stained with Alexa488-streptavidin (left) and Hoechst (DNA, right).