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. 2010 May 10;285(29):22202–22210. doi: 10.1074/jbc.M110.131821

FIGURE 7.

FIGURE 7.

CRM1-C528S rescues nuclear export in the presence of LMB or 15d-PGJ2. A, cells were transfected with plasmids coding for HA-tagged wild-type CRM1 (HA-CRM1-WT) or the CRM1 mutant C528S (HA-CRM1-C528S) as indicated. After treatment with 5 nm LMB or 30 μm 15d-PGJ2 for 1.5 h, cells were fixed and stained with antibodies against RanBP1 and the HA tag. LMB/15d-PGJ2-resistant cells expressing CRM1C528S are indicated by arrows. B, cells were co-transfected with plasmids coding for CRM1 (mutant or wild-type, as indicated) and the reporter protein GFP-TFIIA. After treatment with 5 nm LMB or 15 μm 15d-PGJ2 for 1.5 h, cells were fixed and stained with antibodies against the HA tag. Bars, 10 μm (A) or 5 μm (B). C, quantitative analysis of the subcellular localization of GFP-TFIIA in B. Only cells expressing wild-type HA-CRM or mutant HA-CRM1-C528S were included in the analysis. Error bars indicate the standard deviation from the mean of three independent experiments, counting >100 cells per condition.