TABLE 1.
Plasmids used in this study
| Plasmids | Description | Source |
|---|---|---|
| Gateway cloning vectors | ||
| pENTR1A, -2B and -3C | Gateway entry vectors | Invitrogen |
| pDONR221 | Gateway donor vector | Invitrogen |
| pENTR-EGFP | EGFP without stop codon in Gateway entry vector | (34) |
| pDest15 | Bacterial GST fusion expression vector; T7 promoter | Invitrogen |
| pDEST-TH1 | Bacterial MBP fusion expression vector; tac promoter | (60) |
| pDestEGFP-C1 | Mammalian EGFP fusion expression vector; CMV promoter | (40) |
| pDest-mCherry-C1 | Mammalian mCherry fusion expression vector, backbone as pDestEGFP-C1 | (34) |
| pDest-3xFLAG | Mammalian triple flag tag fusion expression vector; CMV promoter, backbone as p3xFLAG-CMV (Sigma, E4026) | This study |
| pDest-myc | Mammalian myc tag fusion expression vector; backbone as pcDNA3.1 | (40) |
| Other vectors | ||
| pGEX-2T | Bacterial GST fusion expression vector; tac promoter | Amersham |
| pCMV-βgal | Mammalian expression vector for bacterial β-galactosidase; CMV promoter | Open Biosystems |
| pcDNA3.1 | Mammalian expression vector; CMV and T7 promoters | Invitrogen |
| pmCherry-C1 | Mammalian mCherry fusion expression vector, backbone as pEGFP-C1 | (34) |
| Promoter constructs used in reporter gene assays | ||
| pGL3-Basic | Mammalian Luciferase reporter plasmid without promoter or enhancer | Promega |
| Nqo1-ARE-Luc | Murine NAD(P)H:quinine oxidoreductase 1 (Nqo1) promoter (−1016/) with functional ARE subcloned into pGL3-Basic | (61) |
| Nqo1-ΔARE-Luc | Same plasmid as above, but with ARE deleted | (61) |
| pGL3-Pp62(−1781/+46) | Human p62 promoter (−1781/+46) subcloned into pGL3-Basic | (28) |
| pGL3-Pp62(−1475/+46) | Made by deletion of pGL3-Pp62(−1781/+46) | This study |
| pGL3-Pp62(−1158/+46) | Made by deletion of pGL3-Pp62(−1781/+46) | This study |
| pGL3-Pp62(−355/+46) | Made by deletion of pGL3-Pp62(−1781/+46) | This study |
| pGL3-Pp62(−107/+46) | Made by deletion of pGL3-Pp62(−1781/+46) | This study |
| pGL3-Pp62(−1781/+46) Δ(−1464 to −1273) | Made by deletion of pGL3-Pp62(−1781/+46) | This study |
| pGL3-Pp62(−1781/+46) Δ(−1315 to −1273) | Made by deletion of pGL3-Pp62(−1781/+46) | This study |
| pGL3-Pp62(−1781/+46) Δ(−1464 to −1426) | Made by deletion of pGL3-Pp62(−1781/+46) | This study |
| pGL3-Pp62(−1781/+46)-3xMut | Made by site-directed mutagenesis of pGL3-Pp62(−1781/+46); the region from −1302 to −1298 mutated from TGAGT to AGGGA | This study |
| cDNA constructs made by traditional subcloning or site-directed mutagenesis | ||
| pcDNA3-V5-mNrf2 | Mammalian expression vector of murine Nrf2 N-terminally tagged with V5; backbone as pcDNA3.1 | (7) |
| pDONR221-NRF2 | Human NRF2 (from ImaGenes clone IRAUp969G0565D) inserted by gateway BP reaction into pDONR221 | This study |
| pDONR221-MAFG | Human MAFG in Gateway donor vector (HsCD00042726) | DF/HCC DNA Resource Core, Harvard |
| pENTR-KEAP1 | Human KEAP1 (from image clone 3955118) subcloned into pENTR3C | This study |
| pENTR-KEAP1(1–307) | Made by deletion of pENTR-KEAP1 | This study |
| pENTR-KEAP1(308–624) | Made by deletion of pENTR-KEAP1 | This study |
| pENTR-KEAP1 R380A | Made by site-directed mutagenesis of pENTR-KEAP1 | This study |
| pENTR-KEAP1 N382A | Made by site-directed mutagenesis of pENTR-KEAP1 | This study |
| pENTR-KEAP1 R415A | Made by site-directed mutagenesis of pENTR-KEAP1 | This study |
| pENTR-KEAP1 H436A | Made by site-directed mutagenesis of pENTR-KEAP1 | This study |
| pENTR-KEAP1 R483A | Made by site-directed mutagenesis of pENTR-KEAP1 | This study |
| pENTR-KEAP1 Y525A | Made by site-directed mutagenesis of pENTR-KEAP1 | This study |
| pENTR-KEAP1 Y572A | Made by site-directed mutagenesis of pENTR-KEAP1 | This study |
| pENTR-EGFP-KEAP1 | Made by subcloning of KEAP1 into pENTR-EGFP | This study |
| pENTR-p62 | Human p62 in entry vector | (40) |
| pENTR-p62 Δ371–385 | Made by deletion of pENTR-p62 | This study |
| pENTR-p62 D347A | Made by site-directed mutagenesis of pENTR-p62 | This study |
| pENTR-p62 T350A | Made by site-directed mutagenesis of pENTR-p62 | This study |
| pENTR-p62 G351A | Made by site-directed mutagenesis of pENTR-p62 | This study |
| pENTR-p62 E352A | Made by site-directed mutagenesis of pENTR-p62 | This study |
| pENTR-p62 R21A/D69A | p62 R21A/D69A (monomeric) in entry vector | (62) |
| pENTR-p62(321–342) | p62(321–342) in entry vector | (34) |
| pENTR-p62(321–370) | p62(321–370) in entry vector | (34) |
| pENTR-p62(321–358) | Made by deletion of pENTR-p62(321–370) | This study |
| pENTR-p62(339–358) | Made by deletion of pENTR-p62(321–370) | This study |
| pENTR-p62(339–370) | Made by deletion of pENTR-p62(321–370) | This study |
| pENTR-p62 MutNLS | p62 lacking both nuclear location signals made by site-directed mutagenesis of pENTR-p62. Mutations: R186A/K187A/K264A/R265A | This study |
| pENTR-p62 G351A/MutNLS | Made by site-directed mutagenesis of pENTR-p62 MutNLS | This study |
| pENTR-p62(124–440) | p62 ΔPB1 (monomeric) in entry vector | (30) |
| pENTR-p62 W338A/L341A | p62 LIR mutant made by site-directed mutagenesis of pENTR-p62 | This study |
| pENTR-p62 Δ123–319 | Made by deletion of pENTR-p62 | This study |
| cDNA constructs made by Gateway LR reactions (previously published) | ||
| pDest-TH1-p62 | Human p62 in pDest-TH1 | (34) |
| pDest-TH1-p62 Δ123–169 | p62 Δ123–169 in pDest-TH1 | (63) |
| pDest-TH1-p62 Δ170–256 | p62 Δ170–256 in pDest-TH1 | (63) |
| pDest-TH1-p62 Δ256–319 | p62 Δ256–319 in pDest-TH1 | (63) |
| pDest-TH1-p62 Δ256–370 | p62 Δ256–370 in pDest-TH1 | (63) |
| pDest-TH1-p62 Δ386–440 | p62 ΔUBA in pDest-TH1 | (63) |
| pDest15-LC3B | Human LC3B in pDest15 | (34) |
| pDest-myc-p62 | Human p62 in pDest-myc | (40) |
| pDest-myc-p62 R21A/D69A | p62 R21A/D69A in pDest-myc | (62) |
| pDest-myc-p62(1–385) | p62 ΔUBA in pDest-myc | (30) |
| pDest-mCherry-p62 | Human p62 in pDest-mCherry-C1 | (34) |
| pDest-mCherry-EGFP | mCherry-EGFP double tag expression vector | (34) |
| pDest-mCherry-EGFP-LC3B | Human LC3B fused to mCherry-EGFP double tag | (34) |