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. 2010 May 1;285(29):22437–22447. doi: 10.1074/jbc.M110.118984

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© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 3. — Redistribution and interaction between STIM1-CFP and STIM2-YFP induced SOCE activation. A, confocal images of myoblasts co-expressing STIM1-CFP and STIM2-YFP. Cultures were fixed 10 min after the application of 1 μm Tg. The right image shows that STIM1 and STIM2 clusters are nicely co-localized. C, myoblasts were co-transfected 24 h before images acquisition with STIM1-CFP and either STIM1-YFP or STIM2-YFP. The FRET ratio between STIM1-CFP and either STIM2-YFP (upper images) or STIM1-YFP (lower images) before and 10 minutes after 1 μm Tg application are shown. Scale bars represent 10 μm. B, time course of FRET increases between STIM1-CFP and either STIM1-YFP (black) or STIM2-YFP (red) following the application of 1 μm Tg (one representative result out of three independent experiments). Values were normalized to resting FRET before Tg application. The blue curve represents FRET signals in myoblast expressing STIM1-CFP and STIM2-YFP after application of 1 μm DMSO (control). D, Western blot illustrating co-immunoprecipitation of endogenous STIM2 protein using an antibody against STIM1 protein during early differentiation.