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. Author manuscript; available in PMC: 2010 Jul 13.
Published in final edited form as: Endocrinology. 2006 Aug 24;147(12):5975–5987. doi: 10.1210/en.2006-0154

FIG. 2.

FIG. 2

3β-HSD, CYP11A1, and StAR are expressed in male and female brain. A, RT-PCR shows specific amplification of 3β-HSD, CYP11A1, and StAR cDNAs from P1–7 telencephalon (T), diencephalon (D), TeO (O), and cerebellum (C) and adult T, D, and C. B, Northern blots hybridized with 3β-HSD, CYP11A1, and StAR probes confirm the presence of mRNAs in brain. Shown are a male and female brain band from the same age with the exception of 3β-HSD. Three bands were shown for 3β-HSD, from left to right: adult male rest of brain, P1–5 female whole brain, and P1–5 male whole brain; the P1–5 male band is smeared. Gonads from adult male and female serve as positive control. Approximate transcript sizes in kilobases for each band are listed to the left of the gonadal blots for each gene. C, Steroid biochemistry detects 3β-HSD activity in telencephalon (Tel), dien-cephalon (Dien), cerebellum (Cerb), and TeO (Opt) of P5 males and females. Error bars (C), ± sem.