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. Author manuscript; available in PMC: 2010 Jul 13.
Published in final edited form as: Endocrinology. 2006 Aug 24;147(12):5975–5987. doi: 10.1210/en.2006-0154

FIG. 3.

FIG. 3

Adult testes are positive control for in situ hybridization specificity. Testes hybridized with antisense configured probes for 3β-HSD (A and C), CYP11A1 (E and G), and StAR (I and K) show high levels of hybridization over cells patterned as Leydig cells in film images (A, E, and I) and emulsion dipped slides (C, G, and K). No hybridization is seen with sense configured probes (B, F, and J and D, H, and L). Scale bar (A), 1 mm (A, B, E, F, I, and J); scale bar (C), 100 µm (C, D, G, H, K, and L).