The multiple dcn, motor output areas, express CYP17, 3β-HSD, CYP11A1, and StAR. A, Low-magnification film image showing the position of the dcn (collection of darkly stained nuclei in box) in an adult male section hybridized for CYP11A1. B–I, Higher magnification of the area in box. B, D, F, and H, Emulsion-dipped sections hybridized with antisense configured probes for CYP17, 3β-HSD, CYP11A1, and StAR, respectively. The adjacent sections hybridized with sense probes (C, E, G, and I) do not show label. Areas representative of absent (−), medium (++), and high (+++) hybridization levels are indicated. No area determined to have low hybridization is apparent on this section. A vest determined have medium hybridization is marked with an arrow; the dcn and OS, areas of high levels of hybridization, are marked with an arrowhead; and a hindbrain region absent of hybridization is indicated with a tailed arrow. Scale bars (A, B, and H), 1 mm (A, B: B–G, H: H, I).