TABLE 1.

Areas labeled by in situ hybridization in adult (Ad) and P20 brains

	StAR		CYP11A1		3β-HSD			StAR		CYP11A1		3β-HSD
Brain area	Ad	P20	Ad	P20	Ad	P20	Brain area	Ad	P20	Ad	P20	Ad	P20
Telencephalon							Midbrain/hindbrain
HA	+	+	+	+	+	+	HM	−	−	+	+	−	−
M	+	+	+	+	+	+	HL	−	−	+	+	−	−
HD	+	+	+	+	+	++	Tu	−	−	++	++	−	−
MSt	+	+	+	+	+	+	SNc	++	++	++	++	−	−
N	++	++	++	++	+	+	TeO	−	−	++	++	−	−
S	−	−	++	++	++	++	ICo/MLd	++	++	++	++	++	++
TFM	−	−	−	++	−	−	VTA	++	++	++	++	−	−
CoS	+	+	+++	+++	−	−	MPv	−	−	+++	+++	−	−
nCPa	+	+	+++	+++	−	−	IM/IPC	+++	+++	++	++	++	++
HP	+	+	++	++	+++	+++	Pt	−	−	+++	+++	++	++
GP	−	−	+	+	−	−	SRt	−	−	++	++	−	−
LV	−	−	+	+	−	−	SGC	+++	+++	++	++	+++	+++
A	+	+	++	++	++	++	GCt	++	++	++	++	+	+
Tn	−	−	++	++	++	++	III V	−	−	−	−	−	−
Hypothalamus							EM	++	++	+	+	++	++
POA	++	++	++	++	++	++	SLu	++	++	++	++	++	++
PVN	++	++	++	++	++	++	EW	+++	+++	++	++	++	++
VMN	++	++	++	++	++	++	Ru	++	++	+++	+++	++	++
LHy	+	+	+	+	++	++	nOMd/v	+++	++	+++	+++	++	++
Thalamus							mnV	−	−	++	++	−	−
GLv	++	++	++	++	++	++	PL/PM	+++	+++	+++	+++	++	++
LA	++	++	++	++	++	++	VLV	+++	+++	+++	+++	−	−
rot	++	++	++	+++	++	++	RPgc	+++	+++	+++	+++	++	++
DLA	++	++	++	++	++	++	IO	+++	+++	++	++	++	++
DLL	++	++	++	++	++	++	LoC	++	++	++	++	++	++
ICT	++	++	++	++	++	++	OS	+++	+++	+++	+++	−	−
VLT	++	++	++	++	++	++	nPrV	+++	+++	+++	+++	++	++
ov	+++	+++	++	+++	++	++	MV	+++	+++	+++	+++	++	++
RSd/v	−	−	+++	+++	−	−	Dcn	+++	+++	+++	+++	++	++
SPC	++	++	++	++	−	−	Vest	++	++	++	++	++	++
DLP	++	++	++	++	++	++	Song system
DMP	++	++	++	++	−	−	HVC	+	++	+	++	+	+
DLM	++	++	++	++	++	++	RA	+	+	+	++	+	+
PMI	++	++	++	++	++	++	LMAN	++	+	++	+	+	+
DIP	++	++	++	++	++	++	Area X	−	−	+	+	+	+
DP	++	++	−	−	−	−
SpM	+++	+++	+++	+++	++	++
PPC	−	−	++	++	−	−
SP	+++	+++	+++	+++	−	−
SpL	+++	+++	+++	+++	++	++
IS	−	−	++	++	−	−
AP	++	++	++	++	++	++

The hybridization level for each area was subjectively classified as relatively high (+++), medium (++), low (+), or absent (−) based on label intensity observed after film exposure and is intended only as a comparative measure of hybridization levels within an age and gene. Subjective analysis identified brain areas with the highest (+++) and lowest (+) hybridization levels for one gene at one age, then a nucleus of intermediate level (++), to serve as standards for each hybridization level for each gene and age. Brain areas not showing specific label were designated absent (−). The mean hybridization intensity level of each identifiable brain area was subjectively compared with these standard regions on the same film exposure to derive the hybridization level for each gene. No distinction between male and female hybridization is indicated here, and levels are a consensus of all brains at an age hybridized for each gene. MSt, Medial striatum; S, septal nuclei; CoS, commissuralis septi; nCPa, cells of the nucleus commissural pallii; GP, globus pallidus; LV, lateral ventricle; Tn, nucleus taeniae; PVN, paraventricular nucleus; VMN, ventromedial nucleus; LHy, lateral hypothalamus; GLv, lateral geniculate; LA, lateral anterior nucleus; rot, nucleus rotundus; DLA, anterior dorsolateral; DLL, lateral dorsolateral; ICT, nucleus intercalatus; VLT, ventrolateral nucleus; RSd/v, dorsal and ventral superior reticular nuclei; SPC, nucleus superficialis parvocellularis; DLP, posterior dorsolateral; DMP, posterior dorsomedial nucleus; DLM, medial dorsolateral; PMI, paramedianus internis; DIP, posterior dorsointermedius; nucleus PPC, principalis precommissuralis; SP, nucleus subpretectalis; IS, nucleus interstitialis; AP, area pretectalis; Tu, tuberis; SNc, substantia nigra pars compacta; IM, magnocellular isthmus nuclei; IPC, parvocellular isthmus nuclei; Pt, nucleus pretectalis; SRt, nucleus subrotundus; GCt, central gray; EM, ectomammillaris; SLu, nucleus semilunaris; EW, Edinger-Westphal; Ru, nucleus ruber; nOMd/v, distinct cells of the nucleus of the III cranial nerve; PM, medial pons; PL, lateral pons; VLV, ventral portion of the lateral lemniscus; RPgc, gigantocellularis; IO, isthmoopticus; LoC, locus ceruleus; nPrV, principal nucleus of the cranial nerve; MV, motor nucleus of the cranial nerve; N, nidopallium; M, mesopallium; HD, hyperpallium densocel-lulare; HA, hyperpallium apicale; HP, hippocampus; A, arcopallium; LSt, lateral striatum; POA, preoptic area; ov, ovoidalis; SpL, lateral nucleus of the spiriform complex; SpM, medial nucleus of the spiriform complex; SPC, nucleus superficialis parvocellularis; HM, medial habenula; HL, lateral habenula; TeO, optic tectum; SGC, cellular layer stratum griseum cellulare; ICo, nucleus intercollicularis; MLd, dorsal lateral mesencephalic nucleus; VTA, ventral tegmental area; IP, interpeduncularis nucleus; PPT, pedunculopontine tegmental nucleus; dcn, deep cerebellar nuclei; vest, vestibular nuclei; OS, superior olive; PC, Purkinje cells; RA, robust nucleus of the arcopallium; LMAN, lateral magnocellular nucleus of the nidopallium.