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. Author manuscript; available in PMC: 2011 Jul 1.
Published in final edited form as: J Microbiol Methods. 2010 Apr 10;82(1):95–97. doi: 10.1016/j.mimet.2010.03.019

Figure 1. Yersinia pestis colonies isolated from whole fleas using cefsulodin, irgasan, novobiocin (CIN) media.

Figure 1

Triturated flea material was incubated on CIN media for 48 hours (A) or 72 hours (B) at 28°C before presumptive identification. Y. pestis colonies were readily discriminated by colonial morphology of 1-2mm in diameter and bright red with raised fried egg morphology. Panel A shows predominately Y. pestis growth whereas panel B shows Y. pestis and contaminant growth after 72 hours. Arrows in panel B point to Y. pestis colonies.