Table 1.
Comparison of 25(OH)D concentrations measured by the DiaSorin Liaison and HPLC as a result of various exogenous and endogenous treatments.
| Sample ID | DiaSorin Liaison | HPLC |
|---|---|---|
| Total 25(OH)D (ng/ml) | ||
| Baseline | 43.7 ± 16.8 | 48.3 ± 19.0 |
| Vitamin D2 | 81.1 ± 21.9 | 80.0 ± 23.5 |
| 1Baseline + 25(OH)D2 | 51.0 ± 16.8 (22.8%) | 79.7 ± 19.0 (98.3%) |
| 1Baseline + 25(OH)D3 | 63.7 ± 20.4 (62.7%) | 80.0 ± 18.5 (99.0%) |
| Horse Serum | 12.7 ± 1.0 | 4.7 ± 0.2 |
| 2NIST Level 1 [22-24] | 24.4 ± 0.8 (106%) | 26.0 ± 1.1 (113%) |
| 2NIST Level 2 [12-14] | 19.8 ± 0.5 (152%) | 15.9 ± 0.7 (122%) |
| 2NIST Level 3 [42-46] | 27.2 ± 1.0 (61.8%) | 48.1 ± 3.0 (109%) |
1
32 ng/ml was added to each of 9 samples. Values in parentheses represent amount of 25(OH)D recovered as a % of mean values.
2
Values in brackets are expected values provided by NIST. Values in parentheses represent amount of 25(OH)D recovered as a % of mean values.