Figure 1.

Colocalization of GC markers in a subpopulation of cells in the CA3. A, Low-magnification confocal image of CB (top) and Prox1 (middle; Prox1 immunostaining is restricted to the nuclei of positive cells) immunoreactivity. The bottom panel shows the superimposed images of the CB and Prox1 immunostainings (scale bar, 100 μm; asterisks mark MFs in the CA3 area). B, Some CA3 cells are positive to both CB and Prox1 (horizontal arrows). Note the cells immunopositive for CB only (vertical arrowhead). Note also that dendrite-like, CB-positive processes emerge only from the apical side of double-positive cells, whereas CB-positive but Prox1-negative cells have different multipolar somata (scale bar, 20 μm). C, CB and Prox1 staining of DG GCs.