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. 2010 Jul 13;5(7):e11559. doi: 10.1371/journal.pone.0011559

Figure 4. Incomplete DNA replication in myotubes but not fibroblasts reactivated by CKI KD.

Figure 4

(A) MSC-derived myotubes and quiescent C2Q16 myoblasts and C3H-10T1/2 fibroblasts were transfected with siRNAs to p21 and p27 and continuously incubated with BrdU until fixation, 44 hours later. BrdU was detected by indirect immunofluorescence and nuclei were counterstained with Hoechst 33258. Arrowheads point at some incompletely, unevenly BrdU-substituted nuclei. Bar  = 12.5 µm. (B) C2Q16 myotubes and quiescent C2Q16 myoblasts and C3H-10T1/2 fibroblasts were transfected with siRNAs to p21 and p27, stained with propidium iodide, and analyzed by cytofluorimetry at the indicated time points. (C) MSC-derived myotubes and fibroblasts were treated and stained exactly as in (A). Mitotic figures were photographed. Bar  = 25 µm.