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. Author manuscript; available in PMC: 2010 Jul 13.
Published in final edited form as: Biochim Biophys Acta. 2008 Oct 28;1789(2):99–108. doi: 10.1016/j.bbagrm.2008.10.002

Figure 1.

Figure 1

5LO interacts with Dicer C-terminal (C-term) domain in the yeast two-hybrid system. (A–B) Yeast strain PJ69-4A was cotransformed with plasmids expressing the Gal4 BD-5LO and Gal4 AD-human Dicer deletion mutants. (A) Transformants were tested for the Ade, His and LacZ reporter genes. +++, denotes yeast growth visible at 2 days; −, denotes absence of yeast growth at 7 days. Color of the colonies was noted at 7 days. RIII, RNase III motifs; dsRBD, dsRNA binding domain. (B) Expression of the Gal4 AD-human Dicer deletion mutant (upper panel) and the Gal4 BD-5LO (lower panel) proteins was confirmed by immunoblot (IB) analysis.