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. Author manuscript; available in PMC: 2010 Jul 13.
Published in final edited form as: Biochim Biophys Acta. 2008 Oct 28;1789(2):99–108. doi: 10.1016/j.bbagrm.2008.10.002

Figure 2.

Figure 2

Dicer C-terminal domain colocalizes and interacts with 5LO in transiently transfected human cells. (A) Confocal immunofluorescence microscopy of HeLa cells expressing epitope-tagged 5LO and Dicer C-terminal (C-term) proteins. The merged image demonstrates enrichment and colocalization of 5LO and Dicer C-term proteins at the perinuclear region of HeLa cells. Scale bar = 20 μm. (B) Immunoprecipitation experiments using HEK 293 cells expressing epitope-tagged 5LO and Dicer C-term proteins. Proteins present in the cell lysates (5% input) (lanes 1 to 3) and immunoprecipitates (lanes 4 to 6) were detected by immunoblot analysis (IB) using anti-Flag (upper panels) and anti-5LO (lower panels) antibodies.