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. Author manuscript; available in PMC: 2010 Jul 13.
Published in final edited form as: Biochim Biophys Acta. 2008 Oct 28;1789(2):99–108. doi: 10.1016/j.bbagrm.2008.10.002

Figure 5.

Figure 5

5LO interacts with Dicer 5LObd via its N-terminal non-catalytic domain. (A) GST binding assays were performed, as described in the legend of Figure panels 4A and 4B, using 0.5 μg 5LO (lanes 5 and 6) or 5LO W13/75/102A (lanes 2 and 3) recombinant protein. (A, left panel) Bound 5LO protein was analyzed by immunoblotting (IB), and compared with a reference (Ref.) of 5LO (0.05 μg) (lane 1). (A, right panel) 5LO and 5LO W13/75/102A mutant proteins (0.15 μg each), analyzed on the same gel by IB analysis, exhibited similar immunoreactivities (lanes 7 and 8). (B) Immunoprecipitation experiments using HEK 293 cells expressing epitope-tagged Dicer C-term and 5LO or 5LO W13/75/102A mutant proteins. Proteins present in the cell lysates (5% input) (lanes 1 to 4) and immunoprecipitates (lanes 5 to 8) were detected by IB using anti-Flag (upper panels) and anti-5LO (lower panels) antibodies.