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. Author manuscript; available in PMC: 2010 Jul 13.
Published in final edited form as: Biochim Biophys Acta. 2008 Oct 28;1789(2):99–108. doi: 10.1016/j.bbagrm.2008.10.002

Figure 6.

Figure 6

The smallest 5LObd-containing fragment of Dicer exerts stimulatory effects on 5LO activity. (A–C) 5LO enzyme activity assays. Recombinant human 5LO was incubated in the absence or presence of human Dicer 1650-1912 deletion mutant at a 2:1 or 10:1 ratio, or PC, prior to addition to the 5LO activity assays. After incubation for 10 minutes at room temperature, the reactions were stopped and the 5-HPETE and 5-HETE (A), leukotriene (B) formation and total 5LO products (C) were measured by HPLC by using 17-OH-22:4 (A) or prostaglandin B2 (B) as the internal standard.