Skip to main content
. Author manuscript; available in PMC: 2010 Jul 13.
Published in final edited form as: Biochim Biophys Acta. 2008 Oct 28;1789(2):99–108. doi: 10.1016/j.bbagrm.2008.10.002

Table 2.

The amino terminus of 5LO is required for interaction with human Dicer C-terminal (C-term) domain in the yeast two-hybrid system.

Gal4 BD vector Gal4 AD vector Reporter genes
Ade His LacZ
SNF1 SNF4 +++ +++ Blue
Empty Dicer C-term - - White
SNF1 Dicer C-term - - White
5LO Dicer C-term +++ +++ Blue
5LO 62-673 SNF4 - - White
5LO 62-673 Dicer C-term - - White
+++

denotes yeast growth visible at 2 days;

-

denotes absence of yeast growth at 7 days. Color of the colonies was noted at 7 days.