Table 5.
Results of DArT clone redundancy analysis based on DNA sequencing of clones selected from the nine bins that had at least 30 clones per bin, based on Hamming distance of zero (no difference in scoring pattern between markers in the bin)
Bin # | No. of clones per bin based on Hamming distance | No. of clones selected for re-arraying and sequencing | No. of re-arrayed clones with unique DNA sequences | % of re-arrayed clones with unique DNA sequences |
---|---|---|---|---|
1 | 116 | 33 | 19 | 57.6 |
2 | 75 | 20 | 12 | 60 |
3 | 59 | 17 | 8 | 47.1 |
4 | 43 | 13 | 6 | 46.2 |
5 | 41 | 6 | 4 | 66.7 |
6 | 39 | 10 | 5 | 50 |
7 | 37 | 16 | 11 | 68.8 |
8 | 31 | 10 | 6 | 60 |
9 | 30 | 9 | 2 | 22.2 |
Average | 52.3 | 14.8 | 8.1 | 53.2 |