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. 2010 Jul 13;8(7):e1000420. doi: 10.1371/journal.pbio.1000420

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Boehm et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) The right hind limb bud at stage E11.0 from the freshly dissected embryo, and (B) a right hind limb bud from another embryo at stage E11.25, 6 h older than (A). (C) The OPT scans were converted into an iso-surface, a 3D contour of the embryo. The limb bud shapes S_t0, (D) and S_t1 (E) were virtually dissected from the whole-embryo iso-surface. (F) A comparison between the two empirically measured shapes S_t0 (white) and S_t1 (blue) which highlights the shape change over 6 h of development. The main axes are shown anterio-posterior (AP), dorso-ventral (DV), and proximo-distal (PD). (G) A virtual slice through the fully tetrahedtralised S_t0 mesh. The surface is discretised with a triangular mesh (green), and the internal mesenchymal volume is discretised with a 3D tetrahedral mesh (red).