Table 1.
Gene | Primer sequence (5'-3') | |
---|---|---|
Forward | Reverse | |
Inha WT | cct ggg tgg cgc agg ata tgg | ggt ctc ctg cgg ctt tgc gc |
Inha null | cct ggg tgg cgc agg ata tgg | gga tat gcc ctt gac tat aat g |
Amhr2cre | cgc att gtc tga gta ggt gt | gaa acg cag ctc ggc cag c |
Smad2 flox | tac ttg ggg caa tct ttt cg | gtc act ccc tga acc tga ag |
Smad2 null | gct gag tgc cta agt gat agt gca | tct tct ttt tcc ccg ctg g |
Smad2 Rec | gag ctg cgc aga cct tgt tac | gtc act ccc tga acc tga ag |
Rec, recombined floxed allele