Figure 6.

Protective effects against apoptosis after UCF-101 treatment in H₂O₂-induced oxidative stress. (A) x-Axis: JC-1 green, representative of depolarized mitochondria; y-axis: JC-1 red, representative of healthy mitochondria. The results showed that 93.8% of cells stained with JC-1 red but not with JC-1 green, showing that the mitochondria were healthy; 2.81% cells stained both green and red, indicating compromised mitochondria; 2.72% stained green but showed relatively negative red staining, showing total loss of the integrity of the mitochondrial membrane. (B) Only 16.7% cells were with healthy mitochondria after H₂O₂ treatment. As suggested in (C), UCF101 protected ARPE19 cells from H₂O₂-induced apoptosis, 68.7% H₂O₂ + UCF101-treated cells had healthy mitochondria. Moreover, it was preventive but did not rescue the cells.