Fig. 2.

IsoKs induce concentration-dependent dysfunction in nonsynaptosomal brain mitochondria. Mitochondria were challenged with sequential Ca²⁺ additions (5 μM each) in the presence of IsoKs (0.5–2 μM, lines 2–5) until Ca²⁺ was spontaneously released. Mitochondria were incubated with glutamate/malate (5 mM). To induce complete swelling a non specific pore forming agent, alamethicin (Ala) was added at the end of each incubation. Sections are the same as in Fig. 1. Representative traces from 6 experiments are shown. B. Dependence of brain mitochondria Ca²⁺ retention capacity on IsoKs concentration. Data were plotted as the mean ± sem, N=3, *, p<0.05.