Fig. 3.

IsoKs compromise liver mitochondria function in the absence of Ca²⁺. A. Basic mitochondrial parameters were measured in the presence of IsoKs (4 μM). Section a. Mitochondrial ΔΨ was measured as changes in the TMRM fluorescence signal, as described in Fig. 1. Section b. NAD(P)H autofluorescence signal. Section c. Mitochondrial swelling was measured as changes in the fluorescence of the mitochondrial suspension. B. The mitochondrial parameters in the presence of IsoKs (4 μM), ionophore A 23187 (0.5 μg/ml), and EDTA (50 μM). Sections are the same as in panel A. Representative traces from 3 experiments are shown.