Figure 2.

Histological analysis of lens development revealed lens fiber cell differentiation defects in Cryaa-dnNCOA6 lenses. H&E staining was performed with WT (A, C, E, and G) and transgenic (B, D, F, and H) mouse eye sections at E12.5, E15.5, neonatal stage, and P21, respectively, to show lens morphology. At E15.5, the transgenic lens appeared smaller (D) compared with WT (C) and exhibits pyknotic nuclei. Higher magnification images of E15.5 lens fiber cell nuclei are shown as insets (C′ and D′) and pyknotic nuclei are indicated by arrows. The OFZ is located in the center of the WT lens (E and G) and is indicated by dashed circle in E. Transgenic lenses display irregular fiber pattern and pyknotic staining (F and H). The rounded end fragment and cortical liquefaction in dark red are revealed in the inset (H′). Scale bar is shown in each panel.