Figure 5.

Caspase-3 activation in differentiating transgenic lens fiber cells. E13.5 and E14.5 WT and transgenic lenses were subjected to immunofluorescence analysis with CCAP3 antibody. The E13.5 transgenic lens showed staining in the lens fiber cell compartment (D–F) compared with WT (A–C). The amount of CCAP3 staining increased in the E14.5 transgenic lens (J–L) and no staining was detected in the WT lens (G–I). Nuclei (blue signal) were stained with DAPI. Lens epithelium, LE; transitional zone, TZ. Scale bar is shown in panels A, D, G, and J.