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Aminoacylated tRNAs are present in the nucleus of HeLa cells. Total RNA was isolated from nuclear and cytosolic fractions prepared from HeLa cells under acidic conditions. RNA treated with (+) or without (−) base was separated on a 6.5% acid-urea polyacrylamide gel. Northern blot analysis was performed to monitor the aminoacylation status of tRNALys in the cytoplasmic (A) and various tRNA isoacceptors in the nuclear (B) fractions using 32P-labeled oligonucleotide probes.
