Figure 4.

Scyl1 associates specifically with the NPC in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde in PBS and then permeabilized with 0.5% Triton X-100 in PBS (first and third rows) or fixed and permeabilized simultaneously in 2% paraformaldehyde and 0.5% Triton X-100 (second and fourth rows). The location of Scyl1, the NPC and Calnexin was detected by immunofluorescence microscopy. Localization of Scyl1 with the NPC or calnexin was determined by overlay analyses of the micrographs. Insets represent zoomed in regions outlined with white rectangles. Bars, 10 μm.