Figure 7.

Xpo-t, Xpo-5, and Ran but not Crm1 copurify with Scyl1. Total cell lysate from HeLa cells (lane 1) was incubated with Sepharose 4B (lane 2) or α-Scyl1 coupled to Sepharose 4B (lane 3). The resins were washed and boiled in sample buffer to release bound proteins. Western blot analysis was used to detect Xpo-t (first row), Xpo-5 (second row), Ran (third row), Crm1 (fourth row), and Scyl1 (fifth row) in the eluates. The proteins were visualized directly by SYPRO Ruby staining of the blot (sixth row).