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. 2010 Jul 15;21(14):2483–2499. doi: 10.1091/mbc.E10-03-0176

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Figure 9. — Xpo-t interacts directly with RanGTP but not RanGDP in a tRNA-independent manner. GST-Xpo-t (20 μg; 158 pmol) bound to GT-Sepharose in the presence (lanes 3 and 4) or absence (lanes 5 and 6) of an excess of yeast mature tRNA (6 μM) was incubated with Ran (8 μg; 316 pmol) in the GDP (lanes 3 and 5) or RanGppNHp (lanes 4 and 6) form. The resins were washed and boiled in sample buffer to elute bound proteins. GST-Xpo-t (lane 1), Ran (lane 2), and the eluates were subjected to SDS-PAGE, followed by Western blot analyses to detect GST-Xpo-t (top row) and Ran (bottom row).