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. 2010 Jul 15;21(14):2529–2541. doi: 10.1091/mbc.E10-02-0169

Figure 6.

Figure 6.

Expression of the GAT domain disrupts the arrestin-2/STAM-1 interaction and accelerates CXCR4 degradation. (A) Lysates from HeLa cells cotransfected with HA-arrestin-2 and FLAG-STAM-1-GAT (S1-GAT) or empty vector (pCMV) were incubated with anti-HA and IgG control antibodies. Immunoprecipitates were analyzed by immunoblotting to detect bound endogenous STAM-1, and lysates were analyzed to assess expression of the various constructs. Shown are representative blots from one of three independent experiments. (B) HA-CXCR4 degradation was assessed in HEK293 cells expressing FLAG-STAM-1-GAT or empty vector (pCMV) as described in Materials and Methods. (C) Graphical representation of percentage of receptor degraded. Error bars represent SEM from three independent experiments. Data were analyzed by two-way ANOVA and followed by a Bonferroni's post hoc test. (*p < 0.0001). Shown are representative blots from one of three independent experiments.