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. 2010 Jul 15;21(14):2529–2541. doi: 10.1091/mbc.E10-02-0169

Figure 8.

Figure 8.

Expression of Arr2-(25-161) disrupts the STAM-1/arrestin-2 interaction and accelerates CXCR4 degradation. (A) Lysates were prepared from HeLa cells cotransfected with T7-STAM-1, HA-arrestin-2 and increasing amounts (0, 1, and 2.5 μg) of FLAG-Arr2 (25-161). Lysates were divided into equal aliquots and incubated with either an anti-T7 polyclonal antibody or protein G agarose alone (control). Immunoprecipitates were analyzed by immunoblotting to detect bound HA-arrestin-2 and endogenous HRS and lysates were analyzed to assess the expression of the various constructs. Shown are representative blots from one of three independent experiments. (B) HA-CXCR4 degradation was assessed in HEK293 cells expressing FLAG-Arr2-(25-161) or empty vector (pCMV) as described in Materials and Methods. (C) Graphical representation of percent receptor degraded. Error bars represent SEM from three independent experiments. Data were analyzed by two-way ANOVA and followed by a Bonferroni's post hoc test. (*p < 0.0001). Shown are representative blots from one of three independent experiments.