Table 1.
Analyses of tibia performed on day 35 after osteotomy in ovariectomized and intact rats
| Parameter | Ovariectomized | Intact | ||||||
|---|---|---|---|---|---|---|---|---|
| Vibrated | Not vibrated | Vibrate | Not vibrated | |||||
| Mean | SEM | Mean | SEM | Mean | SEM | Mean | SEM | |
| Analyses | ||||||||
| Serum analyses | ||||||||
| Alp (U/l) | 64A | 5 | 63AC | 4 | 47B | 3 | 52BC | 5 |
| Oc (μg/l) | 27 | 4 | 21 | 2 | 19 | 2 | 22 | 2 |
| fpVCT analyses | ||||||||
| Cortical area (mm2) | 0.9A | 0.1 | 0.8A | 0.1 | 1.8B | 0.4 | 2.1B | 0.4 |
| Callus area (mm2) | 11AB | 0.8 | 12A | 1.5 | 8B | 0.8 | 9AB | 1.2 |
| Total area (mm2) | 17 | 0.7 | 16 | 1.4 | 16 | 1.0 | 17 | 0.6 |
| Cortical density (mg/cm3) | 642A | 12 | 655A | 13 | 722B | 21 | 719B | 13 |
| Callus density (mg/cm3) | 54 | 5 | 58 | 6 | 55 | 4 | 59 | 3 |
| Total density (mg/cm3) | 328A | 15 | 334A | 12 | 420B | 27 | 405B | 24 |
| Biomechanical analyses | ||||||||
| Stiffness (N/mm) | 85A | 13 | 122AB | 19 | 78A | 15 | 138B | 30 |
| Yield load (N) | 40 | 6 | 53 | 10 | 40 | 12 | 60 | 17 |
| Microradiography | ||||||||
| Ventromedial aspect | ||||||||
| Cortical width (mm) | 0.5 | 0.03 | 0.5 | 0.02 | 0.4 | 0.03 | 0.4 | 0.1 |
| Cortical density (%) | 99A | 0.2 | 97B | 1 | 100A | 0.1 | 99A | 0.2 |
| Callus width (mm) | 0.3A | 0.03 | 0.3A | 0.02 | 0.4B | 0.1 | 0.4AB | 0.1 |
| Callus density (%) | 81AB | 3 | 72B | 4 | 84A | 4 | 86A | 5 |
| Dorsal aspect | ||||||||
| Cortical width (mm) | 0.6A | 0.03 | 0.7B | 0.03 | 0.5A | 0.02 | 0.5A | 0.02 |
| Cortical density (%) | 98 | 1 | 99 | 1 | 100 | 0.1 | 100 | 0.1 |
| Callus width (mm) | 0.7AB | 0.1 | 0.8A | 0.1 | 0.6C | 0.1 | 0.6BC | 0.04 |
| Callus density (%) | 82AB | 2 | 80B | 3 | 88A | 3 | 87a | 2 |
| Endosteally | ||||||||
| Callus density (%) | 71A | 3 | 68A | 4 | 70A | 7 | 83B | 3 |
| Trabecular nodes (n) | 14A | 3 | 9A | 2 | 39B | 5 | 44B | 3 |
| Trabecular width (μm) | 8 | 3 | 6 | 1 | 8 | 1 | 8 | 0.3 |
Serum analyses and flat-panel volume computed tomography (fpVCT), biomechanical, and microradiographic image analyses of tibia performed at day 35 after osteotomy at the ventromedial and dorsal aspects and endosteally in ovariectomized or intact rats either vibrated or not vibrated. At least nine replications per treatment group were performed. SEM standard error of the mean, Alp alkaline phosphatase, Oc osteocalcin
ABC Between treatment groups means with different superscripts differ (P < 0.05, test)