Table 2.
Osseous callus area (μm2) | Ovariectomized | Intact | ||||||
---|---|---|---|---|---|---|---|---|
Vibrated | Not vibrated | Vibrated | Not vibrated | |||||
Mean | SEM | Mean | SEM | Mean | SEM | Mean | SEM | |
Ventromedial | ||||||||
Total callus, day 35 | 921A | 166 | 951A | 188 | 1622B | 420 | 983AB | 163 |
Callus formation/day | ||||||||
CG | 2x | 1 | 9x | 4 | 12x | 4 | 8x | 3 |
AC | 14Axy | 8 | 20Axy | 4 | 50By | 17 | 30ABy | 6 |
TC | 27y | 7 | 29y | 6 | 45y | 16 | 36y | 5 |
Dorsal | ||||||||
Total callus, day 35 | 4765A | 670 | 5163A | 517 | 3249AB | 528 | 1986B | 190 |
Callus formation/day | ||||||||
CG | 20x | 4 | 24x | 6 | 28x | 6 | 14x | 3 |
AC | 168ABy | 31 | 198Ay | 33 | 91Bxy | 19 | 88By | 15 |
TC | 127Ay | 23 | 137Az | 22 | 101ABy | 22 | 51Bxy | 8 |
Endosteal | ||||||||
Total callus, day 35 | 5780A | 572 | 8511B | 780 | 7347AB | 1154 | 4841A | 601 |
Callus formation/day | ||||||||
CG | 62x | 16 | 97x | 28 | 60x | 20 | 53x | 17 |
AC | 178y | 28 | 175y | 29 | 244y | 55 | 166y | 20 |
TC | 183y | 27 | 185y | 27 | 185y | 36 | 116xy | 15 |
Overall callus | 11487A | 980 | 14590B | 1024 | 12201AB | 1100 | 7834C | 931 |
Callus formation in rats at the ventromedial and dorsal aspects, and endosteally determined 35 days after osteotomy on tibia longitudinal sections labeled intravital with calcein green (CG, 13–18 days), alizarin complexone (AC, 18–26 days), and tetracycline (TC, 26–35 days) in ovariectomized or intact rats either vibrated or not vibrated. At least nine replications per treatment group were performed. SEM standard error of the mean
ABC Between treatment group means with different superscripts differ (P < 0.05, Scheffé test)
xyz Between-labeled callus areas (CG, AC, TC) within a treatment group at the respective tibia aspect means with different letters differ (P < 0.05, Scheffé test)