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. 2010 Jun 9;87(2):168–180. doi: 10.1007/s00223-010-9381-0

Table 2.

Rate of callus formation in ovariectomized or intact rats

Osseous callus area (μm2) Ovariectomized Intact
Vibrated Not vibrated Vibrated Not vibrated
Mean SEM Mean SEM Mean SEM Mean SEM
Ventromedial
 Total callus, day 35 921A 166 951A 188 1622B 420 983AB 163
 Callus formation/day
  CG 2x 1 9x 4 12x 4 8x 3
  AC 14Axy 8 20Axy 4 50By 17 30ABy 6
  TC 27y 7 29y 6 45y 16 36y 5
Dorsal
 Total callus, day 35 4765A 670 5163A 517 3249AB 528 1986B 190
 Callus formation/day
  CG 20x 4 24x 6 28x 6 14x 3
  AC 168ABy 31 198Ay 33 91Bxy 19 88By 15
  TC 127Ay 23 137Az 22 101ABy 22 51Bxy 8
Endosteal
 Total callus, day 35 5780A 572 8511B 780 7347AB 1154 4841A 601
 Callus formation/day
  CG 62x 16 97x 28 60x 20 53x 17
  AC 178y 28 175y 29 244y 55 166y 20
  TC 183y 27 185y 27 185y 36 116xy 15
Overall callus 11487A 980 14590B 1024 12201AB 1100 7834C 931

Callus formation in rats at the ventromedial and dorsal aspects, and endosteally determined 35 days after osteotomy on tibia longitudinal sections labeled intravital with calcein green (CG, 13–18 days), alizarin complexone (AC, 18–26 days), and tetracycline (TC, 26–35 days) in ovariectomized or intact rats either vibrated or not vibrated. At least nine replications per treatment group were performed. SEM standard error of the mean

ABC Between treatment group means with different superscripts differ (P < 0.05, Scheffé test)

xyz Between-labeled callus areas (CG, AC, TC) within a treatment group at the respective tibia aspect means with different letters differ (P < 0.05, Scheffé test)