Fig. 3.
Binding of OxyR to the p M promoter. a Comparison of the efficiency of OxyR binding to p M and oxyR promoter regions. Gel mobility shift assays were performed as described in “Materials and methods” using the 258-bp PCR fragment encompassing pM and the 297-bp fragment of oxyR promoter region. Biding reactions were run on a 5% polyacrylamide gel. b EMSA analysis of OxyR binding to pM promoter region. Gel mobility shift assays were performed as described in “Materials and methods” using 79-bp double-stranded oligos, containing wild-type (lanes 6–10) or mutated sequence (lanes 1–5) of a putative oxyR box. Reactions were run on a 6% polyacrylamide gel