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. Author manuscript; available in PMC: 2010 Jul 14.
Published in final edited form as: J Immunol. 2005 May 1;174(9):5781–5788. doi: 10.4049/jimmunol.174.9.5781

FIGURE 6.

FIGURE 6

Effects of IL-1 and NF-κB inhibition on FN-f- and IL-1β-stimulated cytokine expression. A, Primary human chondrocytes were serum starved overnight and then were treated for 16 h with FN-f (0.5 µM) or IL-1β (2 ng/ml) in the presence of either IL-1ra (100 ng/ml) or the NF-κB inhibitor hypoestoxide (50 µM) in serum-free medium. Samples of conditioned medium were used for immunoblotting with the indicated primary Abs. B, Chondrocytes were treated with FN-f and the inhibitors, as described in A, and RNA was isolated and used for RT-PCR with the indicated primers.