Fig. 1.

Amino acid sequence of IEX-1 (Swiss Prot Acc #P46695) and sites at which the protein might undergo posttranslational modification. The underlined sequence from residue 86 to 111 has homology with rhodopsin-like G protein coupled receptors, and the boxed amino acids from 88 to 93 display the LLXLL motif characteristic of coactivator proteins. A nuclear localization signal, RKRSRR partially overlaps the PKC phosphorylation site. Also shown, in alternating bold and italics, are the tryptic fragments greater than 500 Da, with the monoistopic mass and pl of each unmodified fragment. These fragments represent approximately 93% of the 156-amino acid protein. Calculated molecular weight, 16,927.48 kDa; estimated pl, 8.83