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. Author manuscript; available in PMC: 2011 Feb 1.
Published in final edited form as: Bioanalysis. 2010 Apr;2(4):769–790. doi: 10.4155/bio.10.31

Table 5.

Examples of sandwich immunoassays.

Analyte Detection method Assay characteristics Ref.
Thyroid-stimulating
hormone
Absorbance Range: 0–0.29 nM [154]
hCG Fluorescence Range: 0–66.6 ng/ml [154]
HSA Fluorescence LOD: 0.001 mg/ml [59]
PTH, interleukin-5 Fluorescence LOD: 10 nM; linear range: <250 µM [155]
IgG Absorbance LOD: 3 fmol; range: 3.33–130 fmol [110]
IgG Fluorescence Linear range: 0.5–50 pmol/l [156]
HSA Electrochemical Range: 1–10 mg/ml [157]
Anti-IgG Electrochemical Range: 3–225 fmol [158]
IgG Electrochemical Range: 5–400 ng/ml [159]
IgG Chemiluminescence Range: 0.2–20 fmol [160]
Parathyroid
hormone
Chemiluminescence LOD: 0.24 pM (16 amol);
linear range: 0.24–67 pM
[161]