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. 2010 Aug;51(8):2253–2264. doi: 10.1194/jlr.M005371

Fig. 1.

Fig. 1.

RNAi-mediated silencing of Mttp gene expression in McA-RH cells. A: McA-RH cells were transfected with miR-shRNA-MTP (cassette A or B) or with miR-shRNA-negative control. The mRNA levels of MTP and GAPDH were measured by RT-PCR. B: The levels of MTP protein and β-actin internal control in parental untransfected McA-RH cells and McA-RH cells transfected with miR-shRNA-negative control were determined by Western blot analysis. C: The levels of MTP protein and β-actin internal control in McA-RH cells transfected with miR-shRNA-negative control (lane 1) and several clonal stable transformants of McA-RH cell lines transfected with either miR-shRNA-MTP, cassette A (lanes 2–5) or cassette B (lanes 6–9) were determined by Western blot analysis using antibody to bovine MTP 97 kDa large subunit and antibody to β-actin, respectively.