Fig. 7.

Effects of kinase inhibitors on induction of SHP mRNA in primary rat hepatocytes. Primary rat hepatocytes were prepared without added insulin as described in “Materials and Methods.” After 2 h plating, cells were treated with vehicle, Wortmannin (100 nM), AKTi (1 µM), or PD98059 (50 µM) for 30 min and then TCA (50 μM) for 1, 2, or 3 h. Cells were harvested at 3 h, and mRNA isolated and used for reverse transcription and then for real-time quantitative RT-PCR to quantitate SHP mRNA as described in “Materials and Methods.” ^*P < 0.03, ^**P < 0.079, ^***P < 0.018, NS = not significant. There was no significant difference when mRNA levels were normalized to rGAPDH. Results expressed as % of time 0 control. N = 4. SHP, small heterodimeric partner; TCA, taurocholate.