Fig. 2.

Relationship between lipid rafts and LIF-induced signaling. (A, B) Mouse ES cells were treated with LIF for 0–120 min, and the phosphorylation of STAT3 and Akts (Ser⁴⁷³, Thr³⁰⁸) was detected by Western blot. (C) The cells were incubated for 0–24 h with or without a 1 h preincubation with 10 mM Mβ-CD. c-Myc protein expression was detected by Western blot. (D, E) Cells were transfected for 24 h with a SMARTpool of caveolin-1 siRNA or nontarget control siRNA (0–50 nM) and the phosphorylation of STAT3, Akts, and the expression of c-Myc protein was detected by Western blot. Zero represents no siRNA. Each example is representative of three experiments. The graph denotes the mean ± SE of three experiments for each condition determined from densitometry relative to β-actin. P < 0.05 versus control. ES cell, embryonic stem cell; LIF, leukemia inhibitory factor; Mβ-CD, methyl-β-cyclodextrin; ROD, relative optical density; siRNA, small interfering RNA; STAT3, signal transducer and activator of transcription 3.