Fig. 3.

MiR-122a inhibits CYP7A1 mRNA expression. A–C: HepG2 cells were transfected with the miR-122a mimic (Mir-122) or miR-122a mimic control (Control). mRNAs were isolated for quantitative real-time PCR analysis. The effect of miR-122a mimic on SLC7A1 mRNA expression was assayed as a positive control. D–F: PHHs were used to study the effect of a miR-122a inhibitor (I-Mir-122a) on CYP7A1, CYP8B1, and SLC7A1 mRNA expression. SLC7A1 mRNA expression was assayed as a positive control. Data represent the mean ± SD of at least three individual experiments. ^* P < 0.05 and ^** P < 0.001 indicate statistically significant difference of MiR-122a or I-Mir-122a versus their respective negative control (Control).