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MiR-122a and miR-422a do not play a role in mediating FGF19 inhibition of CYP7A1 mRNA expression in PHH. PHH cells were treated with FGF19 (40 mg/ml) and 100 nM of I-Mir-122a (A), I-Mir-422a (B), or both I-Mir-122a and I-Mir-422a (C) for 6 h. mRNAs were isolated for real time PCR analysis of CYP7A1 mRNA expression levels.
