Fig. 8.

Nuclear run-on assay of FGF19 effects on CYP7A1 mRNA transcription in PHH cells. PHH cells were treated with FGF19 at 40 ng/ml for 24 h. An aliquot of cells were used for total RNA preparation and reverse transcription. The rest of the cells were used for nuclei isolation and nuclear run-on assay as described in the “Materials and Methods” section. The CYP7A1 and CYP8B1 mRNA transcripts from total RNA and run-on assays were measured using real-time PCR and normalized to internal control UBC mRNA. Results were expressed as mean ± SD. N = 3. A P-value of <0.05 is considered as statistical significance.