Fig. 7.

Live imaging of lipid droplets and mitochondria. Myotubes were pretreated for 24 h with 1 µM T0901317 or vehicle (DMSO), and then for another 24 h with 100 µM OA, EPA, LA, PA, 40 µM BSA (control), T0901317, or a combination of T0901317 and EPA. The cells were stained for nuclei (blue), mitochondria (red) and LDs (green) as described in “Materials and Methods.” Representative images are presented for control (A–C), pretreatment with EPA (D–F), and EPA + T0901317 (G–I). Images A, D, and G show mitochondria and nuclei; B, E, and H show LDs and nuclei; and C, F, and I show mitochondria, LDs, and nuclei. Scale bar is 50 µm. EPA, eicosapentaenoic acid; LA, linoleic acid; LD, lipid droplet; OA, oleic acid; PA, palmitic acid; T, T0901317.