TABLE 1.

Number of genes regulated by fatty acid treatment compared with control in myotubes identified by microarray analysis

Treatment	Upregulated Number of Genes	Downregulated Number of Genes
OA	16	20
PA	11	0
LA	20	19
EPA	40	112

Myotubes were incubated with fatty acids (100 µM) or BSA (40 µM, control) for 24 h and harvested for RNA isolation. Gene expression was measured by Affymetrix human NuGO Genechip arrays and fold change (FC) was calculated as log2-transformed expression level after fatty acid treatment divided by log2-transformed expression level after BSA treatment (control) for each individual donor. Mean fold changes were calculated based on the individual log2-transformed fold changes. Differentially expressed probe sets were identified using paired Intensity-based moderated t-statistics (IBMT, q-value) as explained in “Materials and Methods.” Genes with q < 0.2 was considered significantly regulated. Abbreviations: EPA, eicosapentaenoic acid; LA, linoleic acid; OA, oleic acid; PA, palmitic acid.