Fig. 9.

Immunohistochemistry of HMGR, the rate-limiting enzyme in cholesterol synthesis, in corpora lutea from WT (A, C) or SCARB1^−/− mature mice (B, D) stimulated with eCG and hCG and collected 18 h after hCG. Mice received placebo injections (A, B) or a combination of 20 µg/g BW mevinolin (Mev; an HMGR inhibitor) and 100 µg/g BW chloroquine (Chloro; an inhibitor of lysosomal processing of LDL) (C, D). A insert: Negative control (NC) for the SCARB1 immunofluorescence staining reaction where the first antibody was omitted. Bars: 50 µm. eCG, equine chorionic gonadotropin; hCG, human chorionic gonadotropin; HMGR, 3-hydroxy-3-methylglutaryl CoA reductase; SCARB1, scavenger receptor-B1; WT, wild type.